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Abstract #10029 Published in IGR 5-3
The neuroprotective effect of erigeron breviscapus (vant) hand-mazz on retinal ganglion cells after optic nerve crush injury
Jiang B; Jiang YChinese Journal of Ophthalmology 2003; 39: 481-484
OBJECTIVE: To investigate whether a Chinese herbal medicine, ergeron breviscapus (vant) hand-mazz (EBHM), can protect the retinal ganglion cells (RGC) damaged by calibrated optic nerve crush injury. METHODS: Forty-two Sprague-Dawley rats were randomly divided into two groups. A calibrated optic nerve crush model was induced into the right eyes by a specially designed optic nerve clip. The left eyes served as a control. Group A consisted of rats with calibrated optic nerve crush injury; group B consisted of rats with calibrated optic nerve crush injury treated with EBHM. In group B, EBHM solution was given once after the crush injury. According to the time interval between optic nerve crush and sacrifice, both groups A and B were further divided into three subgroups (day 4, day 14, and day 21). Therefore, there were seven rats in each subgroup. Three days before sacrifice, 3% fast blue was injected into the superior colliculi bilaterally. The eyes were enucleated after the rats had been sacrificed, and flat mounts of the retinas from both eyes were prepared on a slide and observed under a fluorescence microscope. Four photos with 400x magnification were taken of each of the four quadrants of the retina, 1 mm away from the optic disc. The labeled RGC were counted by means of a computerized image analyzer. The labeled RGC rate was used for statistical analysis (the labeled RGC rate = number of RGC in injured eye/control eye x100%). RESULTS: In group A, the labeled RGC rate was 77.79 ± 7.11, 63.76 ± 3.79, and 54.66 ± 4.75% on days 4, 14, and 21, respectively. In group B, the labeled RGC rate was 80.13 ± 12.03, 78.17 ± 9.19, and 83.59 ± 12.61% on days 4, 14, and 21, respectively. In group B, which was treated with EBHM after injury, the labeled RGC rate was significantly higher than that in group A on days 14 and 21. CONCLUSIONS: In this experimental optic nerve crush model in rats, EBHM therapy could increase the survival rate of RGC and could rescue and/or restore the injured RGC. LA: Chinese
Dr. B. Jiang, Department of Ophthalmology, The Second XiangYa Hospital, Sun yat-sen University, Changsha 410011, China. youqin3403@yahoo.com.cn
Classification:
11.8 Neuroprotection (Part of: 11 Medical treatment)
