advertisement
Abstract #10044 Published in IGR 6-1
Expression of estrogen receptor alpha and 17beta-hydroxysteroid dehydrogenase 4 in the ciliary body
Kobayashi K; Iwakiri R; Kobayashi H; Okinami SGraefe's Archive for Clinical and Experimental Ophthalmology 2004; 242: 172-176
PURPOSE: To investigate the expression and distribution of estrogen receptor alpha (ERα) and 17β-hydroxysteroid dehydrogenase 4 (HSD4) in bovine and monkey ciliary body. METHODS: Immunohistochemical study was used to investigate the expression of ERα and 17β-HSD4. To detect ERα and 17β-HSD4 transcripts, reverse transcription-polymerase chain reaction (RT-PCR) was used. RESULTS: In bovine and monkey ciliary body, the staining of the anti-ERα antibody was distributed in nonpigmented epithelium, and was not detectable in pigmented epithelium. Vessels in the stroma were also stained. The expression of 17β-HSD4 was observed in nonpigmented and pigmented epithelium. RT-PCR revealed that the transcripts of ERα and 17β-HSD4 were expressed in monkey ciliary body. CONCLUSIONS: This study provides evidence for the presence of ERα and 17β-HSD4 in the ciliary body. The presence of ERα and 17β-HSD4 suggests that estrogen may have important functions in the ciliary body.
Dr. K. Kobayashi, Department of Ophthalmology, Saga Medical School, 5-1-1 Nabeshima, 849-8501 Saga, Japan. kobayask@post.saga-med.ac.jp
Classification:
2.9 Ciliary body (Part of: 2 Anatomical structures in glaucoma)