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Abstract #10189 Published in IGR 6-1
Effect of various anti-glaucoma eyedrops on human corneal epithelial cells
Aoyama Y; Motoki M; Hashimoto MNippon Ganka Gakkai Zasshi 2004; 108: 75-83
PURPOSE: To investigate the effects of antiglaucoma eye drops and vehicles on the proliferation of human corneal epithelial cells. MATERIAL AND METHODS: Seven eye drops (prostaglandin F2 alpha analogues (two), beta blockers (40), topical carbonic anhydrase inhibitor (one)), and six of the eye drop vehicles, excluding that of Xalatan, were used. Anti-glaucoma eye drops and vehicles were serially diluted two-fold with culture medium (10-2560 fold). The mixture was added to human corneal epithelial cells and incubated for 48 hours. Cell proliferation was measured by commercial assay kit. Dye reagents were added to the wells and incubated for one hour at 37°C. Optical density were measured at 490 nm. The dilution rate for 50% inhibition was calculated as the dilution rate of drugs or vehicles necessary to produce 50% inhibition of cell proliferation. RESULTS: All drugs completely inhibited cell proliferation when the dilution rate was low. At 40-fold dilution, Trusopt and Timoptol showed a significant decrease in cell growth inhibition. On the other hand, Rescula showed almost 100% inhibition at 160-fold dilution. Above 640-fold dilution, the inhibition rate of all drugs became 50% or less and there was no significant difference between drugs. Vehicles also inhibited cell growth. The dilution rates for growth inhibition by vehicles were different from those of drugs. The dilution rate at 50% inhibition of anti-glaucoma eye drops decreased in the following order: Rescula > Xalatan > Betoptic > Hypadil > Mikelan > Timoptol > Trusopt. The dilution rate for 50% inhibition of vehicles decreased in the following order: Rescula vehicle > Hypadil vehicle > Betoptic vehicle > Mikelan vehicle > Timoptol vehicle > Trusopt vehicle. CONCLUSIONS: All anti-glaucoma eye drops inhibited cell proliferation. These effects were stronger in prostaglandin F2 alpha analogues and weakest in Trusopt. Furthermore, the inhibition of cell proliferation was caused also by the vehicle of eye drops, and the influence of the vehicle varied in each type of eye drops. LA: Japanese
Dr. Y. Aoyama, Department of Ophthalmology, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki 216-8511, Japan
Classification:
12.20 Other (Part of: 12 Surgical treatment)