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Abstract #10200 Published in IGR 6-1
In vitro release of mitomycin C from collagen implants
Zimmerman C; Drewe J; Flammer J; Shaarawy TCurrent Eye Research 2004; 28: 1-4
PURPOSE: To study the behavior of mitomycin C loaded collagen implant (CI) pharmacokinetics in vitro. METHODS: CIs were incubated for 15 minutes in different MMC loading solutions at the following concentrations: 0.03 (n = 9), 0.3 (n = 10) and 3.0 (n = 10) mg/ml. The loaded CI were transferred in 100 μl of 0.9% NaCl. Aqueous flow of 5 μl/min was simulated. MMC concentration of the samples was determined by high performance liquid chromatography (HPLC). Dissolution kinetics were evaluated by a first-order process. The half-life of dissolution and the time of 95% dissolution were determined. RESULTS: On average, CI absorbed an MMC dose of 0.054, 0.530 and 6.090 μg when incubated in the different MMC loading solutions containing 0.03, 0.3, and 3.0 mg/ml of MMC, respectively. In the release experiments, the mean total dose delivered by CI was 0.0493, 0.585 and 5.291 μg. A linear correlation between loading concentration and the estimated total dose released was demonstrated. The kinetic parameters showed a fast MMC dissolution. The half-life of the three series was 8.8, 10.1 and 10.5 minutes, respectively. CONCLUSIONS: Commercially available CI can be loaded with MMC, and could provide relatively slower release than sponge delivery of MMC. The clinical implication of these results warrants further studies.
Dr. C. Zimmerman, Department of Clinical Pharmacology, University Hospital, Basel, Switzerland
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)