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Abstract #10290 Published in IGR 6-1
Increased expression of matrix metalloproteinases in mononuclear blood cells of normal-tension glaucoma patients
Golubnitschaja O; Yeghiazaryan K; Liu R; Monkemann H; Leppert D; Schild H; Haefliger IO; Flammer JJournal of Glaucoma 2004; 13: 66-72
PURPOSE: Glaucomatous optic neuropathy involves tissue remodeling by matrix metalloproteinases (MMPs). In this study, the authors investigated MMP gene expression in circulating leukocytes isolated from normal tension glaucoma (NTG) patients in comparison to healthy controls. METHODS: Blood samples were collected from six glaucoma patients and six age- and sex-matched healthy controls. Leukocytes were separated using Ficoll-Hypaque gradient. mRNA pools were used for subtractive hybridization to identify genes with altered expression. The subtracted genes were sequenced and individual mRNA pools were quantified using semiquantitative RT-PCRs. Target PCR products were confirmed using sequence-based restriction analysis. The authors focused on MMPs. RESULTS: MMP-9 and MT1-MMP (MMP-14) were subtracted as upregulated genes in the group of NTG patients. Upregulation of these genes was confirmed by RT-PCR and Western-blot analysis in all six patients. Expression of the tissue inhibitor of matrix metalloproteinases TIMP-1 was slightly enhanced in patients as compared with controls. Expression of MMP-2 was not detected in leukocytes either in glaucoma patients or in healthy subjects. CONCLUSIONS: The simultaneous upregulation of both MMP-9 and MT1-MMP gene expression and only slightly enhanced expression of TIMP-1 suggest an increased enzymatic matrix metalloproteinase activity delivered by mononuclear blood cells in these patients.
Dr. O. Golubnitschaja, Department of Radiology, University of Bonn, Bonn, Germany
Classification:
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
9.4.15 Glaucoma in relation to systemic disease (Part of: 9 Clinical forms of glaucomas > 9.4 Glaucomas associated with other ocular and systemic disorders)