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Abstract #10502 Published in IGR 6-1
Anti-proliferative effect of amnion implant in glaucoma filtering surgery in rabbit
Song Y; Zhang Y; Li D; Cui Y; Yang L; Wang Y; Sun DChinese Ophthalmic Research 2004; 22: 13-16
OBJECTIVE: To study whether amnion implant can prevent and inhibit fibroblast proliferation and scar formation during glaucomatous filtrating surgery in rabbit eyes. METHODS: Glycerin-stored human amnion with epithelium outside were surgically inserted under the scleral flaps of rabbit eyes in an experimental group during trabeculectomy procedures. HE staining, light microscope and microphoto-analysis techniques were used to observe fibroblast proliferation and scar formation at different time points postoperatively. RESULTS: Fibroblast were revealed in the filtrating pathway during the second postoperative week, numbers of fibroblasts were generally increased, but there was no statistic difference at different time points (p > 0.05). Severe fibroblast proliferation and randomly arranged fibrocytes existed in the control group, with partial filtrating tunnel closure during the first week post-surgery. By the second week postoperatively, large amounts of fibroblast filtration and scar formation were found at the tunnels with almost complete closure of filtrating pathways in the control group. The numbers of fibroblasts in the experimental and control groups were compared during the first, second, third and fourth weeks post-surgery, and showed significant statistic differences (p < 0.05). CONCLUSIONS: Amnion can be successfully used for subscleral flaps in anti-glaucomatous filtrating surgery to prevent fibroblast proliferation and scar formation and to keep filtrating pathways open. LA: Chinese
Dr. Y. Song, Department of Ophthalmology, Second Clinical Hospital, Jilin University, Changchun 130041, China
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
