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Abstract #10516 Published in IGR 6-1
Expression of CNTF and CNTFRα mRNA in retina after optic nerve injury in rats
Zou Q; Ye J; Zhu P; Wang Z; Li ZChinese Ophthalmic Research 2004; 22: 54-56
OBJECTIVE: To explore the expressions of ciliary neurotrophic factor (CNTF) and ciliary neurotrophic factor recepter α (CNTFRα) mRNA in the retina after optic nerve injury. METHODS: An optic nerve injury model in rats was created by crushing the optic nerve at 5 mm retrobulbarly. The RT-PCR technique was applied to detect mRNAs of CNTF and CNTFRα in retina one, three, seven, 14 and 28 days postoperatively. RESULTS: mRNA of CNTF and CNTFRα were expressed in normal retina. Levels of CNTF mRNA were significantly increased on days 1, 3, 7, 14 and 28 after injury (p = 0.00). The level of CNTFRα mRNA was obviously increased at days 1, 3, 7 and 14 after injury (P = 0.00). CONCLUSIONS: The increased expression of CNTF and CNTFRα mRNA in retina after optic nerve injury suggests that CNTF and CNTFRα mRNA are likely to protect the degenerative processes of nerve cells induced by optic nerve injury in rat retina. LA: Chinese
Dr. Q. Zou, Department of Ophthalmology, Institute of Field Surgery Research, Third Military Medical University, Chongqing 400042, China
Classification:
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
5 Experimental glaucoma; animal models
11.8 Neuroprotection (Part of: 11 Medical treatment)
