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Abstract #11816 Published in IGR 7-1
Effects of elevated intraocular pressure on mouse retinal ganglion cells
Ji J; Chang P; Pennesi ME; Yang Z; Zhang J; Li-D; Wu SM; Gross RLVision Research 2005; 45: 169-179
We developed and characterized a mouse model of elevated intraocular pressure (IOP) to investigate the underlying cellular and genetic mechanisms of retinal ganglion cell (RGC) death. IOP was unilaterally increased in C57BL/6J mice by photocoagulation of the episcleral and limbal veins. IOP was measured using an indentation tonometer. RGC survival was measured by retrograde labeling using DiI applied to the superior colliculous. The mechanism of RGC death was investigated using TUNEL staining, immunostaining for cleaved caspase-3, and Western blot for Bcl-2 and Bax expression. RT-PCR was used to measure changes in Bcl-2, Bax, Bad, Bak, P53, ICE and Fas. Mean IOP was increased in the treated eyes from 13 ± 1.8 to 20.0 ± 2.8 mmHg at four weeks and 17 ± 2.2 mmHg at eight weeks. RGC loss was 15.6 ± 3.4% at two weeks and 27.3 ± 4.5% at four weeks after laser photocoagulation. TUNEL staining and caspase-3 positive cells were increased in the ganglion cell layer (GCL) in the treated eyes and seldom found in the control eyes. Bcl-2 expression in control group was higher than in the experimental group, while Bax expression in the control group was less than in experimental group. This mouse model resulted in a consistent, sustained increase in IOP with a reduction in the number of RGCs in the treated eye. The RGCs in eyes with elevated IOP were TUNEL-positive, with increased caspase-3 and decreased Bcl-2, consistent with apoptosis as the mechanism of neuronal cell death.
Dr. J. Ji, The Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, 6565 Fannin, NC-205, Houston, TX 77030, USA
Classification:
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
5 Experimental glaucoma; animal models
