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Abstract #118376 Published in IGR 24-4
Protection against Oxidative Stress by Coenzyme Q10 in a Porcine Retinal Degeneration Model
Deppe L; Deppe L; Mueller-Buehl AM; Tsai T; Erb C; Dick HB; Joachim SCJournal of personalized medicine 2024; 14:
Oxidative stress plays an important role in neurodegenerative diseases, including glaucoma. Therefore, we analyzed if the antioxidant coenzyme Q10 (CoQ10), which is also commercially available, can prevent retinal degeneration induced by hydrogen peroxide (HO) in a porcine organ culture model. Retinal explants were cultivated for eight days, and HO (500 µM, 3 h) induced the oxidative damage. CoQ10 therapy was applied (700 µM, 48 h). Retinal ganglion cells (RGCs) and microglia were examined immunohistologically in all groups (control, HO, HO + CoQ10). Cellular, oxidative, and inflammatory genes were quantified via RT-qPCR. Strong RGC loss was observed with HO ( ≤ 0.001). CoQ10 elicited RGC protection compared to the damaged group at a histological ( ≤ 0.001) and mRNA level. We detected more microglia cells with HO, but CoQ10 reduced this effect ( = 0.004). Cellular protection genes () against oxidative stress were stimulated by CoQ10 ( ≤ 0.001). Furthermore, mitochondrial oxidative stress () increased through HO ( = 0.038), and CoQ10 reduced it to control level. Our novel results indicate neuroprotection via CoQ10 in porcine retina organ cultures. In particular, CoQ10 appears to protect RGCs by potentially inhibiting apoptosis-related pathways, activating intracellular protection and reducing mitochondrial stress.
Experimental Eye Research Institute, University Eye Hospital, Ruhr-University Bochum, In der Schornau 23-25, 44892 Bochum, Germany.
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