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Abstract #12064 Published in IGR 7-1
Experimental high IOP model and hypertension-induced lipid hyperoxygen reaction
Wang X; Yin W; Ding X; Tian X; Pang G; Li J; Han XChinese Ophthalmic Research 2004; 22: 620-622
OBJECTIVE: To creat a acute high intraocular pressure (IOP) model and investigate experimental hypertension-induced lipid hyperoxygen reaction in ocular tissue in rabbits. METHODS: Twenty-six rabbits were randomly divided into α-Chymotrypsin group and 2% Methylcellulose group. High IOP was induced by the anterior chamber injection of above two drugs. IOP, electroretimogram (ERG) and optical nerve fiber thickness were examined, and NOS activity and MDA level in lens, retina and choroidal homogeneous plasma were measured. RESULTS: A hypertension model was successfully established in experimental rabbits. IOP was (31.59 ± 6.49) and (37.59 ± 11.41) mmHg in two groups, respectively. Loss of retinal ganglion cell was revealed from histologic examination. Biochemical parameters showed that there was a slight increase in NOS activity. NO and MDA levels were significantly increased in lens, chloroid and retina (P < 0.05). Change of these biochemical parameters was positive correlation with retinal ganglion cell apoptosis of glaucoma. CONCLUSION: High IOP can induce NO and MDA, suggesting that it is necessary to treatment of glaucoma with antioxidate drug. LA: Chinese
Dr. X. Wang, Henan Institute of Ophthalmology, Zhengzhou 450003, China
Classification:
5 Experimental glaucoma; animal models
