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Abstract #12196 Published in IGR 7-2
Induction of TGF-β1 on proliferation of Tenon's capsule fibroblasts and expression of connective tissue growth factor
Huang M; Guo H; Wu J; Chen J; Zhao SChinese Ophthalmic Research 2005; 23: 272-275
OBJECTIVE: To investigate the induction of transforming growth factor β1 (TGF-β1 ) on the proliferation of human Tenon's capsule fibroblasts and expression of connective tissue growth factor(CTGF) in vitro. METHODS: The effects of TGF-β1 on the proliferation of Tenon's capsule fibroblasts was investigated using MTT colorimetry. The 3-6 passage Tenon's capsule fibroblasts were incubated with 4 different concentrations of TGF-β1 (0, 0.1, 1, 10 ng/mL) in 0.5% FBS + DMEM medium. The expression of CTGF was detected by immunocytochemistry. Imaging and statistical analysis was used to evaluate the induction of CTGF. RESULTS: Absorbent value of 0, 0.1, 1, 10 ng/mL of TGF-β1 was 0.079 ± 0.005, 0.110 ± 0.011, 0.121 ± 0.008 and 0.123 ± 0.009 respectively, showing a promotion on the proliferation of Tenon's capsule fibroblasts with the proliferation rate 39.2%-55.7%. The positive index of 0, 0.1, 1, 10 ng/mL of TGF-β1 was 27.74 ± 8.32, 86.12 ± 21.44, 109.46 ± 27.62 and 115.53 ± 32.78, respectively, indicating a induction on the expression of CTGF with significant intergroup differences (P < 0.05, P < 0.01), except for 1 ng/mL group vs 10 ng/mL group (P > 0.05). CONCLUSION: TGF-β1 can enhance Tenon' s capsule fibroblasts' proliferation and induce Tenon's capsule fibroblasts to express CTGF in a dose-dependent manner, inferring that CTGF may play an important role in scarring after glaucoma filtering surgery acting as a downstream mediator of TGF-β1 action. LA: Chinese
Dr. M. Huang, Department of Ophthalmology, Medical College of Jinan University, Guangzhou 510632, China.
Classification:
3 Laboratory methods
2.5.1 Trabecular meshwork (Part of: 2 Anatomical structures in glaucoma > 2.5 Meshwork)
