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Abstract #12331 Published in IGR 7-2
Expression of bFGF receptor and TGF-β receptors in cultured human scleral fibroblasts
Qu J; Li H; Zhou XT; Hu DN; Zhang LH; Fu XY; Lu FChinese Journal of Ophthalmology 2005; 41: 464-467
OBJECTIVE: To examine the expression of basic fibroblast growth factor receptor (FGF R1) and transforming growth factor-β receptors (TGF-β RI and TGF-β RII) in cultured human scleral fibroblasts. METHODS: Scleral fibroblasts of passages 2-4 were used for the present studies. Polyclonal antibodies against FGF R1, TGF-β RI and TGF-β RII were used to detect the proteins of these receptors. Indirect immunoflurescene staining method (IIF) was used. RESULTS: Antibodies for FGF R1, TGF-β RI and TGF-β RII produced specific staining of the entire cell surface, including the cell membrane enveloping cytoplasmic projections; positive staining in some cells was most intensive in the perinuclear region. Immunostaining mainly originated from the cell membranes, indicating that the presence of the receptor proteins on the cell surface. The intensity of staining for TGF-β RI and TGF-β RII was relatively strong, while staining of FGF R1 was relatively weak. The cells treated with PBS instead of primary antibodies did not produce specific staining. CONCLUSION: This study shows that cultured human scleral fibroblasts express the receptor protein for FGF R1, TGF-β RI and TGF-β RII and also indicates that these growth factors may influence these cells. Exogenous bFGF and TGF-β administration may elicit actions through the binding with these receptors in the scleral fibroblasts. LA: Chinese
Dr. J. Qu, School of Optometry and Ophthalmology, Wenzhou Medical College, Wenzhou 325003, China
Classification:
2.3 Sclera (Part of: 2 Anatomical structures in glaucoma)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)