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Abstract #13162 Published in IGR 7-3
Noninvasive intraocular pressure measurements in mice by pneumotonometry
Avila MY; Munera A; Guzman A; Do CW; Wang Z; Stone RA; Civan MMInvestigative Ophthalmology and Visual Science 2005; 46: 3274-3280
PURPOSE: To develop a reliable, noninvasive, continuous, and easily implemented system for measuring intraocular pressure (IOP) in mice. METHODS: Pneumotonometry was adapted for measurement of mouse IOP. Measurements were compared with those obtained with the servo-null micropipette system (SNMS) and with direct anterior chamber cannulation. Heart rate was monitored by the precordial pulse, EKG, or tail pulse in anesthetized mice. The characteristic ocular hypotensive response to mannitol was assessed as an additional validation of the METHOD: RESULTS: Measurements of IOP obtained using pneumotonometry agreed closely with values measured by SNMS or by direct cannulation. IOP oscillations were synchronous with the heart rate, with a coherence peak between them of approximately 2 Hz, equal to the pulse frequency. Hypertonic mannitol reduced IOP from 13.7 ± 0.9 mmHg by 7.7 ± 0.7 mmHg after 15 minutes. CONCLUSIONS: Pneumotonometry is a reliable and noninvasive method for the measurement of IOP in mice and may permit comparisons of IOP to hemodynamic factors. This system is simpler and more adaptable for glaucoma research than previously reported methodologies for measuring IOP in mice.
Dr. M.Y. Avila, Department of Physiological Sciences, Facultad de Medicina, Universidad Nacional de Colombia, Bogota, Colombia
Classification:
6.1 Intraocular pressure measurement; factors affecting IOP (Part of: 6 Clinical examination methods)
5 Experimental glaucoma; animal models
