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Abstract #13298 Published in IGR 8-1
Differential expression of leucocyte common antigen CD45 by microglia in the glaucomatous optic nerve
Chew SH; Penfold PLAustralian Journal of Medical Science 2005; 26: 87-91
The aim of this present study is to establish whether the same population of resident microglial cells in glaucomatous retina was also present in its associated optic nerve. Formalin-fixed specimens of optic nerve segments with glaucoma were processed for paraffin histology and immunohistochemistry. Two morphological forms of CD45 expressing cells were observed in both normal control and glaucomatous optic nerve resembling the ramified parenchymal cells and rounded perivascular cells reported in previous studies of glaucomatous retina. Although the perivascular cells remained fairly constant, a significantly greater number of ramified parenchymal cells were observed in glaucomatous optic nerve compared with normal optic nerve. This study has shown in human optic nerve that there is an increase in resident microglia of leucocyte lineage possibly caused by the pathological changes of glaucoma.
Dr. S.H. Chew, School of Biomedical Sciences, Division of Health Sciences, Curtin University of Technology, Perth, WA 6102, USA
Classification:
2.15 Optic nerve (Part of: 2 Anatomical structures in glaucoma)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
3.1 Microscopy (Part of: 3 Laboratory methods)