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Abstract #13463 Published in IGR 8-1
Preliminary study of inhibition of human Tenon's capsule fibroblasts in vitro by RNA interference targeting IKK-β
Huang SS; Ge J; Wang LN; Yin XB; Wei YT; Ma PChinese Journal of Ophthalmology 2005; 41: 1076-1081
OBJECTIVE: To determine whether small interference RNA (siRNA) targeting the inhibitor of kappaB kinase-β (IKK-β) could be used to suppress the IKK-β expression, and inhibit the proliferation of human Tenon's capsule fibrolasts in vitro. METHODS: IKK-β specific siRNA designed from the human gene sequence was transfected into the cultured human Tenon's capsule fibroblasts, and a non-targeted siRNA was transfected as a negative control. The mRNA of IKK-β was assessed by reverse transcription-polymerase chain reaction (RT-PCR), and the protein expression was determined by Western blotting. Cell viability of the cultured human Tenon's capsule fibroblasts with series of RNA interference at 5, 10, 25, 50, 100, and 200 nmol/L was evaluated by MTT assay 48 hours after transfection. RESULTS: The expression of IKK-β was significantly (P < 0.05) suppressed at both mRNA and protein levels after transfection. The proliferation of the cultured human Tenon's capsule fibroblasts was inhibited at all the transfected concentrations at different rates (10.72%, 23.35%, 30.84%, 51.25%, 50.06% and 49.63% respectively). The highest level of inhibition was observed at 50 nmol/L of siRNA concentration. CONCLUSIONS: IKK-β specific siRNA is effective in suppressing the IKK-β expression and inhibiting the proliferation of the cultured human Tenon's capsule fibrolasts. RNA interference may offer a new alternative to post-operational management of scar tissue formation. LA: Chinese
Dr. S.S. Huang, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, China
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
