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Abstract #13569 Published in IGR 8-2
Distribution regularity of intraocular pressure of normal rats under pentobarbital anesthesia
Ma JZ; Xie L; He XG; Sun YLChinese Journal of Clinical Rehabilitation 2005; 9: 115-117
AIM: To observe the value of the normal intraocular pressure (IOP), and detect the distribution regularity in the daytime and the fluctuation in the day and night of rats so as to establish normal parameter standard and provide reference for establishment of glaucoma model and visual functional rehabilitation. METHODS: The experiment was conducted in the Institute of Surgery, Duping Hospital, the Third Military Medical University of Chinese PLA between October 2004 and April 2005. One hundred and five healthy SD rats, weighing 150-350 g, of either sex were enrolled to measure the value of IOP. Another 20 healthy SD rats, weighing 175-225 g, of either sex were enrolled to measure the day/night fluctuation. All rats underwent measurement of IOP by using Tono-PenXL ophthalmotonomete at the same time points after general anesthesia via intraperitoneal injection of 10 g/L pentobarbital (IOP: 6:00-20:00, recorded twice; Fluctuation: 6:00-7:00, 11:00-12:00, 15:00-16:00, 19:00-20:00, each recorded twice and continuously for four days). RESULTS: Normal IOP was measured in 105 rats, and five rats died from over-anesthesia. Finally, 400 IOP values were obtained. Rats in the fluctuation test were all involved in the result analysis. 1. The results of K-S examination showed that IOP of rats was in the Gauss's distribution, and the mean value was 18.09 ± 3.87 mmHg (1 mmHg = 0.133 kPa), the 95% range of the mean IOP was 17.71-18.45 mmHg (K-S value = 1.20, P = 0.112), and that of the normal IOP at 6:00-7:00 was 11.99-24.15 mmHg. 2. The results of one-way analysis of variance indicated that there were significant differences among the four time phases (F = 22.36, P value approaching to 0). The mean value of IOP at 6:00-7:00 was 16.17 ± 2.74 mmHg, significantly lower than those at 11:00-12:00, 15:00-16:00, and 19:00-20:00 17-51 ± 3.41 mmHg, 18.24 ± 2.45 mmHg, 19.65 ± 3.24 mmHg, P < 0.05. IOP at 19:00-20:00 was the highest with the difference of 3.5 mmHg approximately. CONCLUSION: The normal IOP in rats presents Gauss's distribution with the same fluctuation in the day and night as human being, which is lower in the morning, then elevates gradually till the evening higher than in the morning followed by a decrease to the basic value. LA: Chinese
Dr. X.-G. He, Department of Ophthalmology, Research Institute of Surgery, Third Military Medical University of Chinese PLA, Chongqing 400042, China
Classification:
5 Experimental glaucoma; animal models
6.1 Intraocular pressure measurement; factors affecting IOP (Part of: 6 Clinical examination methods)
12.17 Anesthesia (Part of: 12 Surgical treatment)
