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Abstract #13577 Published in IGR 8-2
Histopathologic study of endoscopic photocoagulation
Shi X; Pang X; Li B; Sun YChinese Ophthalmic Research 2006; 24: 54-57
OBJECTIVE: To investigate the morphological changes of ciliary processes after endoscopic cyclophotocoagulation (ECT) in rabbit eyes. METHODS: Twenty pigmented rabbits received endoscopic cyclophotocoagulation with diode laser. Microscopic structure and ultrastructure of ciliary processes were observed less than 30 minutes and at 3, 7, 28, 90 days after cyclophotocoagulation under the light and electron microscope. RESULTS: After the endolaser photocoagulation was performed, the damage of ciliary processes included ciliary epithelia thinning and vascular congestion of the stroma immediately. The numbers of cysts were increased in the pigmented and nonpigmented ciliary epithelia with the survival time. The nonpigmented epithelium was collapsed or thinned, and the pigment clumped and stromal edema was alleviated. By month 3 after laser treatment, the degree of damage was more pronounced. The ciliary processes were shortened and exhibited fusion in the ciliary body. Transmission electron microscopic study showed edema and vacuolae of mitochondria crests in the nonpigmented epithelium immediately after cyclophotocoagulation and appeared dissolution chromatin of capillary endothelial cells nucleus three days later. Three months after cyclophotocoagulation, mitochondria dissolved, and the cell composition was thrown into confusion. No regeneration of the ciliary processes was found. CONCLUSION: ECT can induce irreversible damage of ciliary processes in the pigmented rabbits without injury of other ocular tissue. The procedure provides a safe and an effective method for the treatment of glaucoma. LA: Chinese
Dr. X. Shi, Beijing Tongren Eye Center, Capital University of Medical Sciences, Beijing 100730, China
Classification:
12.10 Cyclodestruction (Part of: 12 Surgical treatment)
