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Abstract #14220 Published in IGR 8-3
Characteristics of Aquaporin-1 expression in trabecular meshwork and iris
Xiong X; Xi Z; Miao J; Zhang H; Wei HChinese Ophthalmic Research 2006; 24: 303-305
OBJECTIVE: Studies have proved that many ocular diseases are associated with the activity of Aquaporin-1. These diseases include cataract, glaucoma and central retinochroiopathy. This study was to observe the characteristics of Aquaporin-1 (AQP-1) expression in trabecular meshwork and iris. METHODS: Spacemen of trabecular meshwork and iris were collected during the trabeculectomy from the patients with glaucoma. Expression of Aquaporin-1 in trabecular meshwork and iris was detected by immunohistochemistry and compared with normal ocular tissues from donors. RESULTS: The positive staining with a strong brown yellow color was seen in the cell membrane and cytoplasma of normal trabecular meshwork and Schlemm's canal endothelia, while a relatively weaker positive staining was also visible in the same sites of primary angle-closure and open-angle glaucoma sufferers. There was a positive staining in both stroma and epithelium of periphery iris in spacemen from normal and glaucomous eyes, however, in part samples of acute angle-closure glaucoma, the expression of AQP-1 was weaker compared with normal spacemen. The corneal basement epithelium possessed a denser positive staining than the superficial cells. CONCLUSION: There is different exertion of AQP-1 in some ocular tissues in normal and glaucomatous patients. The reduction of AQP-1 expression in trabecular meshwork of primary open-angle glaucoma may be associated with its developing process. The low expression of AQP-1 in iris epithelia and trabecular meshwork of acute-angle closure glaucoma may be the results of hypertension and iris atrophy caused by attack. LA: Chinese
Dr. X. Xiong, Department of Ophthalmology, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China
Classification:
2.5.1 Trabecular meshwork (Part of: 2 Anatomical structures in glaucoma > 2.5 Meshwork)
2.8 Iris (Part of: 2 Anatomical structures in glaucoma)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
