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Abstract #15118 Published in IGR 8-4
Dosage and kinetics of MMC release of a collagen implant used as a delivery device in glaucoma surgery in the rabbit eye
Adjadj E; Roy S; Zimmermann C; Shaarawy T; Flammer J; Mermoud A; Drewe JJournal Français d'Ophtalmologie 2006; 29: 1042-1046
PURPOSE: To determine the absorption and the release of mitomycin-C from a collagen implant and tissue impregnation in the anterior structures of the rabbit eye. METHODS: Determining the quantity of mitomycin-C that a collagen implant can absorb with the difference between dry and soaked weight. Mitomycin-C release was measured in vitro using spectrophotometry. The measurement was repeated using a bioassay. Ocular tissue impregnation was determined in 12 eyes of six rabbits. Sclera, implant, aqueous, and ciliary body specimens were collected for concentration measurement using HPLC from 1 to 6 h after surgery. RESULTS: The mean mitomycin-C quantity absorbed in the implant was 3.22 ± 0.2 μg. In vitro release was 0.13 mg/ml after 10 min and 0.05 μg/ml at 6 h. The bioassay showed almost no antifibrotic activity in sclera. In vivo release of mitomycin-C was high from the first to the sixth hour. CONCLUSION: After filtering surgery, mitomycin-C in the collagen implant is clearly released and ocular tissues are effectively impregnated. LA: French
Dr. E. Adjadj, Hopital Ophtalmique Jules Gonin, Universite de Lausanne, Switzerland
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
5 Experimental glaucoma; animal models
