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Abstract #15353 Published in IGR 1-3
Expression of integrin receptors in the human trabecular meshwork
Zhou L; Maruyama I; Li Y; Cheng L; Yue BCurrent Eye Research 1999; 5: 395-402
PURPOSE: To examine the expression of integrin receptors in the human trabecular meshwork. METHODS: The expression of integrins in human tissues was visualized by immunohistochemical staining using integrin-specific antibodies. Immunoprecipitation was performed after biotin labelling of cell surface proteins. Reverse transcriptase-polymerase chain reaction was used to detect the presence of mRNA species for integrin subunits. RESULTS: Human trabecular meshwork tissues obtained from donors aged from two to 65 years, stained positively for integrins α1, α3, α4, α5, α6, av, β1, β3, β4 and β5. The staining was observed mostly around the edges of trabecular beams in association with trabecular meshwork cells. The staining intensity did not appear to vary with donor age. In addition, immunoprecipitation of tissue extracts revealed the presence of integrin α2 and confirmed the absence of β2. Results from polymerase chain reactions were consistent with these findings. CONCLUSIONS: A spectrum of integrin receptors that may have important roles in the cell-matrix interactions is demonstrated in the human trabecular meshwork. The repertoire identified in tissues is similar to that found in cultured cells except that the β4 expression in tissues is lost in cultures.
Dr. L. Zhou, University of Illinois at Chicago College of Medicine, Department of Ophthalmology and Visual Sciences, Chicago, IL: USA
Classification:
2.5 Meshwork (Part of: 2 Anatomical structures in glaucoma)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)