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Abstract #15733 Published in IGR 2-3
Modulation of pre-mRNA splicing and protein production of fibronectin by TGF-β2 in porcine trabecular cells
Li J; Tripathi BJ; Tripathi RCInvestigative Ophthalmology and Visual Science 2000; 41: 3437-3443
PURPOSE: To determine the effect of transforming growth factor (TGF)-β2 on the pre-mRNA splicing pattern of fibronectin, as well as on the synthesis and secretion of this glycoprotein by porcine trabecular cells. METHODS: First-passage porcine trabecular cells were rendered quiescent and incubated in culture medium containing 15% newborn calf serum, in serum-free culture medium containing either activated TGF-β2 (concentration range: 0.2-2.7 ng/ml) or activated TGF-β1 (1 ng/ml), or in serum-free medium alone (untreated control samples). For investigation of alternative splicing, total RNA was extracted, and reverse transcription-polymerase chain reaction (RT-PCR) was performed with primer pairs located in exons flanking the exon (extra domain (ED)A, or EDB) that undergoes alternative splicing. The polymerase chain reaction (PCR) products were verified by Southern hybridization and quantified by using laser densitometry. The percentage of EDA-positive (+) isoforms was compared with that of the EDB+ isoforms among the groups. To study the effect of TGF-β2 on the synthesis and secretion of fibronectin, total protein was extracted from both cultured cells and conditioned medium, Western blot analysis was performed using an anti-fibronectin antibody, and the products were quantified by laser densitometry. Immunocytochemical analysis was also performed on cultured trabecular cells to detect fibronectin. RESULTS: Fibronectin mRNA that was detected in untreated serum-starved control cells was EDA and EDB negative. Incubation of trabecular cells in medium containing 1 ng/ml TGF-β2, 1 ng/ml TGF-β1, or 15% newborn calf serum induced the expression of EDA+ and EDB+ mRNA to varying degrees. At concentrations of 0.2, 0.5, 1.5, and 2.7 ng/ml, TGF-β2 increased the concentration of fibronectin two-, three-, 3.8-, and five-fold in the conditioned medium, and three-, 3.7-, four-, and 4.3-fold in the cell extracts, respectively. The trabecular cells treated with TGF-β2 exhibited strong immunoreaction for fibronectin, whereas the cells incubated in serum-free medium showed only minimal immunoreactivity. CONCLUSIONS: These results demonstrate that TGF-β2 and TGF-β1 modified the alternative splicing pattern of fibronectin pre-mRNA and enhanced the synthesis and secretion of this extracellular matrix molecule by trabecular cells in a dose-dependent fashion. These findings indicate a mechanism whereby TGF-β2, the concentration of which is elevated in aqueous humor of patients with primary open-angle glaucoma, contributes to the increased deposition of extracellular matrix molecules in the outflow pathway.
Dr. J. Li, Departments of Ophthalmology and Pathology, University of South Carolina School of Medicine, Columbia, SC 29209, USA
Classification:
2.5 Meshwork (Part of: 2 Anatomical structures in glaucoma)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
4 Tissue culture of ocular cells
