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Abstract #15757 Published in IGR 2-3
Lesion-induced regulation of netrin receptors and modification of netrin-1 expression in the retina of fish and grafted rats
Petrausch B; Jung M; Leppert CA; Stürmer CAMolecular and Cellular Neurosciences 2000; 16: 350-364
To determine whether netrin receptors (DCC, UNC5H1, UNC5H2) and netrin-1 are present in the adult rat retina and may affect regeneration of retinal ganglion cell (RGC) axons into peripheral nerve (PN) grafts, in situ hybridization (ISH) and immunostaining experiments were performed in normal and operated rats. Netrin-1 expression was not found in the optic nerve head of adult rats, normal and postlesion, but appeared transiently at seven and 14 days after PN grafting. ISH signals of netrin receptors, however, disappeared from RGCs within two days after lesion and remained absent after PN grafting except for UNC5H2, which transiently occurred in a few RGCs. Netrin-1 expression was observed in the optic nerve head of adult fish, normal and postlesion, and the netrin-1 Fc fusion protein bound to young growing and all regenerating axons. Thus, the netrin-1-dependent guidance system continues to function in fish but apparently no longer operates in adult rats.
Dr. B. Petrausch, Department of Biology, University of Konstanz, Germany
Classification:
11.8 Neuroprotection (Part of: 11 Medical treatment)
