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Abstract #15886 Published in IGR 2-3
Multidrug resistance-associated proteins in glaucoma surgery
Esser J; Esser P; Mietz H; Hüber A; Kociok N; Schraermeyer U; Krieglstein GKGraefe's Archive for Clinical and Experimental Ophthalmology 2000; 238: 727-732
BACKGROUND: Multidrug resistance (MDR) is the phenomenon of cross-resistance between different cystostatic agents which are structurally and functionally dissimilar. Two recently discovered proteins, lung resistance protein (LRP) and the multidrug resistance-related protein (MRP), have been implicated in the development of MDR. Since resistance to chemotherapeutic agents is a common problem in filtration surgery, especially in cases of complicated glaucoma, the authors decided to investigate the presence of MRP and LRP in surgically removed tenon specimens from glaucoma patients. METHODS: The presence of MRP and LRP in surgically removed tenon tissue (n = 15) was analyzed by immunohistochemistry. The expression by cultured tenon fibroblasts was assessed by reverse-transcriptase polymerase chain reaction (RT-PCR) and fluorocytometry. RESULTS: LRP expression was detected in eight of ten tenon specimens. Positive staining for MRP was obtained in five of ten specimens. Negative controls with non-immune mouse IgG did not display any specific staining. RT-PCR and fluorocytometry revealed constitutive expression of MRP and LRP, at the RNA and protein level respectively, which was unaltered by pretreatment of the cells with mitomycin C or 5-fluorouracil. CONCLUSIONS: These results demonstrate that besides P-glycoprotein, other components of the MDR system are present in conjunctival fibroblasts. Future developments in the use of chemotherapeutic agents in association with of filtration surgery should take account of the presence of these counteracting mechanisms.
P. Esser, University Eye Clinic Cologne, Joseph-Stelzmann-Strasse 9, 50931 Cologne, Germany
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
