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Abstract #16005 Published in IGR 2-3
Pressure influence on mRNA expression and protein synthesis of inducible nitric oxide synthetase in bovine trabecular meshwork cells
Xue W; Du S; Li YChinese Journal of Ophthalmology 2000; 36: 295
OBJECTIVE: To investigate the inducible nitric oxide synthetase (iNOS) mRNA expression and protein synthesis in bovine trabecular meshwork cells, and to discuss the possible effects of nitric oxide (NO) on the development of glaucoma. METHODS: Twenty, 40, 60, and 80 mmHg pressure was respectively added to cultured trabecular cells of newborn cows. No pressure group was set as a control. The changes in iNOS mRNA and protein in the trabecular meshwork cells under different pressures were demonstrated qualitatively and quantitatively by in situ hybridization and nicotinamide-adenine dinucleotide phosphate diaphorase (NADPH-d) histochemical assay. RESULTS: The expression of iNOS mRNA and its protein synthesis rose more and more as the pressure was increased. There was weak iNOS mRNA expression in the control and 20 mmHg groups, with no significant difference between them. The 60 and 60 mmHg groups showed a statistical difference from the control group, and the 80 mmHg group showed a very significant difference from the control group. There was no difference between the 40 and 60 mmHg groups, but they were significantly different from the 80 mmHg group. NADPH-d histochemical assay showed almost the same results, except that there was a significant difference between the 40 and 60 mmHg groups. CONCLUSIONS: Pressure can evoke the expression of iNOS mRNA, and the nitric oxide thus produced can be one of the causes of trabecular meshwork destruction that may induce or aggravate glaucoma.LA: Chinese
Dr. W. Xue, Department of Ophthalmology, Tongji Hospital, Tongji Medical University, Wuhan 430030, China. xuewei@public.wuhan.cngb.com
Classification:
2.5 Meshwork (Part of: 2 Anatomical structures in glaucoma)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
