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Abstract #17148 Published in IGR 9-1
Influences of hyperbaric oxygenation therapy on cytochrome oxidase and nitric oxide synthase in retina of rats with experimentally elevated intraocular pressure
Yi J-H; Peng Z-R; Liu Q-L; Wu Z-QChinese Journal of Clinical Rehabilitation 2006; 10: 114-116
AIM: To observe the promotion of hyperbaric oxygenation therapy on cytochrome oxidase (CO) and nitric oxide synthase (NOS) in rats with experimentally acute elevated inlraocular pressure (IOP). METHODS: The experiment was done in the Department of Hyperbaric Oxygenation, Xiangya Hospital, Central South University and the Laboratory of Anatomy, Xiangya Medical College, Central South University from March to August 2005. Twenty-five healthy Wistar rats were divided into 3 groups randomly: 1 Model group included nine rats. Acute elevated IOP models were built after b wave disappearing for 90 minutes by perfusion of saline. No other therapy was done after establishing models. 2 Hyperbaric oxygenation group included 9 rats. After establishing elevated IOP models, the rats received 0.2 MPa hyperbaric oxygenation therapy and sucked in oxygen of high concentration for 80 minutes, average volume fraction of 0.825 2 ± 0.025 8, once a day for 7 times. 3 Seven rats in the blank control group only received keratonyxis. Retina of both sides was measured after experiment. Quantitative analysis of NOS and CO was performed with HPIAS-1000 computer image analysis, including positive cell population, absorbance (A), bulk density and mean section area of positive product. Correlation analysis was also conducted. RESULTS: Totally 25 rats were involved in the result analysis. 1 NADPH/ NOS positive cell population, A, bulk density and mean section area of positive product in the hyperbaric oxygenation group were higher than those in the model group [(13.67 ± 2.40), (5.00 ± 2.92) pieces per field;0.137 ± 0.015, 0.088 ± 0.028; (0.52 ± 0.12)%, (0.22±0.12)%; (74.00 ± 4.61), (63.44 ± 7.02) μm2 ;P < 0.01]. 2 CO positive cell population, A, bulk density and mean section area of positive product in the hyperbaric oxygenation group were higher than those in the model group [(13.44 ± 3.05), (4.67 ± 2.12) pieces per field; 0.123(±)0.026,0.089 ± 0.035; (0.859 ± 0.30)%, (0.182 ± 0.068)%; (99.89 ± 6.05), (68.78(±)10.16) μm2 ;P < 0.05]. 3 The four indexes of activities of NOS and CO showed positive correlation after treatment (P < 0.01). CONCLUSION: Hyperbaric oxygenation therapy can increase the activities of NOS and CO in ischemic tissues of retina. It indicates that hyperbaric oxygenation can protect cranial nerve cells against ischemic injures. LA: Chinese
Dr. J.-H. Yi, Department of Ophthalmology, Third Xiangya Hospital, Central South University, Changsha 410013 Hunan Province, China
Classification:
11.8 Neuroprotection (Part of: 11 Medical treatment)
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
