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Abstract #18973 Published in IGR 3-1
Pattern-electroretinographic changes in the DBA/2NNia mouse-model of angle-closure glaucoma (pilot study)
Bayer AU; Brodie SE; Mittag TOphthalmologe 2001; 98: 248-252
PURPOSE: The aim of this study was to document the time-course of retinal dysfunction by pattern-electroretinography (PERG) in eyes of the DBA/2NNia substrain of mouse that develop an inherited angle-closure glaucoma. METHODS: Twelve DBA/2NNia mice and 12 control C57BL/6 J mice were studied by PERG recordings from two to ten months of age. PERGs were recorded using different spatial and temporal frequencies. Results. PERGs recorded with a temporal frequency of 7.5 Hz and a spatial frequency of 0.4 cycles/degree performed best to discriminate between DBA/2NNia mice and C57BL/6 J mice. When compared with normal C57BL/6 J mice, significant amplitude reductions of the PERG (Student's t test; p < 0.01) were found in DBA/2NNia mice by five months of age and continued to decline as the animals aged. At start of follow-up, the mean PERG amplitude in DBA/2NNia mice was 2.3 ± 0.5 μV. At five months of age, the mean PERG amplitude was reduced by 0.9 ± 0.45 μV (paired t test; p < 0.0001). CONCLUSIONS: Previously, a significant loss of retinal ganglion cells was found in the DBA/2NNia mouse substrain at six to seven months of age. In the present study, the authors found decreases in PERG amplitudes, occurring from the age of five months onward. Similarities with the findings in human glaucoma indicate the relevance of this animal model for further glaucoma research. LA: German
Dr. A.U. Bayer, Department of Ophthalmology, Mount Sinai Medical Center, 1 Gustave L. Levy Place, New York, NY 10029, USA
Classification:
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
