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Abstract #19056 Published in IGR 3-1
A study on the competitive binding test of glucocorticoid receptor in patients with glucocorticoid-induced glaucoma
Mingkai L; Jian G; Yehong Zet al.Chinese Ophthalmic Research 2000; 18: 442-444
OBJECTIVE: To defined the characteristics of glucocorticoid receptor (GR) by radiological binding assays and to develop a new approach to treat glucocorticoid-induced glaucoma with respect to receptor. METHODS: Competitive binding tests were taken with Ru38486 (mifepristone) to evaluate the values of IC50 (inhibited 50% specific binding) and KI (inhibition constant) of GR in peripheral blood lymphocytes from nonglaucomatous subjects and patients with glucocorticoid-induced glaucoma. RESULTS: The tests showed that KI and IC50 of GR of peripheral blood lymphocytes were (44. 56 ± 5.10) nmol/l and (224.30 ± 18.79) nmol/l in patients with glucocorticoid-induced glaucoma and (37.88 ± 2.79) nmol/l and (195. 99 ± 16.08) nmol/l in normal persons. The difference (KI, IC50) between the patients and normal persons was significant (p < 0.05). CONCLUSIONS: The competitive test indicates that the peripheral blood lymphocytes of patients with glucocorticoid-induced glaucoma have more GR numbers than normal subjects. Ru38486 could block GR competitively and therefore has a potential to be developed as a new drug to treat glucocorticoid-induced glaucoma and other glaucomas. LA: Chinese
Dr L. Mingkai, Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China
Classification:
9.4.1 Steroid-induced glaucoma (Part of: 9 Clinical forms of glaucomas > 9.4 Glaucomas associated with other ocular and systemic disorders)
