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Abstract #19339 Published in IGR 9-3
Relationship of cytokines levels and optic nerve damage in open-angle glaucoma patients
Huang P; Qi Y; Xu Y; Zhang CChinese Ophthalmic Research 2007; 25: 454-457
OBJECTIVE: Researches proved that the apoptosis of retinal ganglion cells is a vital pathway to cause optic neuropathy in primary open-angle glaucoma(POAG), while many cytokines were responsible for cell apoptosis. The present study was to investigate the relationship of sIL-2R, IL-2, IL-4, IL-6, IL-12 and IFN(gamma) levels in serum to different stages of glaucomatous neuropathy in POAG patients. METHODS: In this study, 82 patients with POAG(glaucoma group) and 29 normal individuals (control group) were collected. Eighty-two eyes of 82 POAG were divided into 3 groups according to the mean defect (MD) of visual fields (group A: MD < 6 dB; group B: 6 dB(less-than or equal to)SMD <12 dB; group C: MD≥12 dB). Cytokines such as sIL-2R, IL-2, IL-4, IL-6, IL-12 and IFN(gamma) in serum were assayed by Enzyme-linked immunosorbent assay (ELISA) and the mean concentrations of these factors in different groups were analyzed and compared. RESULTS: The levels of sIL-2R and IL-4 in glaucoma group were significantly elevated in comparison with control group (t = 2. 084, P = 0. 045; t = 2. 229, P = 0.030, respectively); the levels of IL-6 and IL-12 in serum were reduced in glaucoma patients compared with normal individuals(t = -2.640, P = 0.011; t = -4. 05.P = 0.000, respectively). There were no significant differences in IL-2 and IFN(gamma) serum concentrations between two groups(t =0. 055, P = 0.956; t = -1.517, P = 0.138, respectively). In POAG patients, the level of sIL-2R in group A (69.519 ± 25.557 pg/ml) was significantly lower than that in group C (89.452 ± 24.051 pg/ml) (P = 0.018), and the concentration of IL-12 in group A(113.097 ± 43.309 pg/ml) was significantly higher than that in group C(81.816 ± 43.814 pg/ml) (P =0.025). However, no statistical significant differences were found in other cytokines among the different stage of diseases. CONCLUSION: Cellular immune system may be different in POAG patients from normal person. Cytokines such as sIL-2R, IL-2, IL-4, IL-6, IL-12 may be associated with glaucomatous optic neuropathy. LA: Chinese
Dr. P. Huang, Department of Ophthalmology, Third Hospital of Peking University, Peking University Eye Center, Beijing 100083, China
Classification:
3.10 Immunobiology (Part of: 3 Laboratory methods)