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Abstract #19341 Published in IGR 9-3
Effects of low dose of latrunculin-A on actin cytoskeleton and extracellular matrix protein TSP-1 in dexamethasone-induced bovine TMCs
Cao J; Zhang DChinese Ophthalmic Research 2007; 25: 502-505
OBJECTIVE: Glaucoma is the leading cause of irreversible blindness. Now there is no specifically effective treatment against it in clinic. We determined the effects of a low dose of the actin-disrupting agent latrunculin (LAT)-A on dexamethasone (DEX) -induced changes in actin organization and extracellular matrix proteins in cultured bovine trabecular meshwork cells(BTMCs). METHODS: BTMCs were primarily cultured with tissue method and subcultured to the 3rd passage. The 3rd generation of cells were divided into untreated group, DEX treated for 2 weeks group, DEX for 2 weeks + LAT-A for 2 hours group and DEX for 1 week + LAT-A for 1 week group. The marking of F-actin in the cell was performed by FITC-phallodin and observed under the Olympus fluorescence microscope. The expression of LAT-A was detected by immunohistochemistry. RESULTS: The primarily cultured BTMCs showed variable appearance, but the 3rd passage of BTMCs had stable shape with framework and affluent cytoplasm, and these cells inosculated as confluent monolayer like flat epithelium. The 3rd passage of cells were identified by immunohistochemistry. The cells treated with DEX showed actin cytoskeletal reorganization and formation of cross-linked actin networks. In DEX treated cells, actin fiber bundles were highly concentrated at the periphery of the cell with few actin filaments in the central area. After LAT-A treatment, there was mild disorganization of actin cytoskeleton, and actin staining in DMSO treated cells was comparable to untreated cells. CONCLUSION: This study suggests that LAT-A at low dose can avoid the ocular high pressure induced by DEX. It may be useful in the treatment and prevention of steroid and other glaucoma. LA: Chinese
Dr. J. Cao, Department of Ophthalmology, Affiliated First Hospital, Xi'an Jiaotong University, Xi'an 710061, China. jan981629@163.com
Classification:
11.14 Investigational drugs; pharmacological experiments (Part of: 11 Medical treatment)
9.4.1 Steroid-induced glaucoma (Part of: 9 Clinical forms of glaucomas > 9.4 Glaucomas associated with other ocular and systemic disorders)
