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Abstract #19420 Published in IGR 9-3
Regulatory effect of dexamethasone on aquaporin-1 expression in cultured bovine trabecular meshwork cells
Xiong X; Miao J; Xi Z; Zhang H; Han B; Hu YJournal of Huazhong University of Science and Technology. Medical sciences 2005; 25: 735-737
To evaluate the effect of dexamethasone on the expression of aquaporin-1 (AQP-1) in cultured bovine trabecular meshwork cells, bovine trabecular meshwork cells were cultured in vitro and reproduced to the third and the fourth generation, then treated with dexamethasone at the concentrations of 5, 25, 50, 250 microg/L respectively for 7 days. Immunohistochemical technique-supervision method was employed to measure, and image analysis system to analyze the expression of AQP-1 in normal cultured bovine trabecular meshwork cells and those treated with dexamethasone. In normal bovine trabecular meshwork cells, the grayscale of AQP-1 positive staining was 167.94 ± 1.18, while it was 168.92 ± 0.91, 176.72 ± 1.80, 180.64 ± 1.31, 185.64 ± 1.58 in cells treated with 5, 25, 50, 250 microg/L concentrations of dexamethasone. When the concentration of dexamethasone was higher than 25 microg/L, the expression of AQP-1 was significantly inhibited (P < 0.05). The regulation of AQP-1 expression by dexamethasone in cultured bovine trabecular meshwork cells in vitro may be one of causes that retard the aqueous outflow in glucocorticoid- induced glaucoma.
Dr. X. Xiong, Department of Ophthalmology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China
Classification:
2.5.1 Trabecular meshwork (Part of: 2 Anatomical structures in glaucoma > 2.5 Meshwork)
3.8 Pharmacology (Part of: 3 Laboratory methods)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
