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Abstract #19567 Published in IGR 9-3
The effect of ICG on mitomycin C cytotoxicity in human tenon fibroblasts
Reeves G; Wallis R; Crowston JG; Small KM; Wells APJournal of Glaucoma 2007; 16: 479-482
PURPOSE: To examine the effects of indocyanine green (ICG) with and without mitomycin C (MMC) on proliferation of cultured human Tenon fibroblasts. METHOD: Fibroblast monolayers were exposed to either MMC [0.4 mg/mL in phosphate buffered saline (PBS)] or PBS containing ICG (0.0625%, 0.125%, 0.25%, and 0.5% in 200 microL PBS) or a combination of MMC (0.4 mg/mL in PBS) and ICG (0.25% and 0.5%) for 5 minutes. Controls were exposed for 5 minutes to MMC, PBS, or culture medium containing no ICG. After treatment, the monolayers were washed and incubated in culture medium for 24, 48, 72 hours, and 1 week periods after which the number of viable cells was quantified. RESULTS: The presence of ICG alone, at concentrations ranging from 0.0625% to 0.5%, had no effect on the rate of fibroblast proliferation measured at any of the incubation periods. As expected, MMC treatment resulted in a significant reduction in viable fibroblast number (8.4 ± 0.13 x 10(3)). ICG in combination with MMC did not significantly alter fibroblast numbers (8.5 ± 0.05 x 10(3)) up to 1 week compared with MMC alone (8.4 ± 0.12 x 10(3)). CONCLUSIONS: ICG at concentrations of 0.5% and below do not reduce proliferation of Tenon capsule fibroblasts. ICG did not potentiate or diminish the effect of MMC on Tenon capsule fibroblast proliferation.
Dr. G. Reeves, Ophthalmology Unit, Department of Surgery, Wellington School of Medicine, Wellington, New Zealand
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
