advertisement
Abstract #19687 Published in IGR 9-4
Expression of neuronal nitric oxide synthase in the retina of a rat model of chronic glaucoma
Park SH; Kim JH; Kim YH; Park CKVision Research 2007; 47: 2732-2740
We investigated the expression of neuronal nitric oxide synthase (nNOS) in a rat retina model of chronic glaucoma, which was produced by electrocauterization of the episcleral vessels. Western-blot analysis showed that nNOS expression was significantly increased in cauterized retinas. nNOS immunoreactivity was observed in the cells of both the inner nuclear layer and the ganglion cell layer. Double labeling of retinal ganglion cells (RGCs) revealed that RGCs in the retina of cauterized rat was nNOS-immunopositive. Systemic administration of l-NAME (N(G)-nitro-l-arginine-methyl-ester), a non-specific NOS inhibitor, reduced RGC loss in cauterized rat retina, but there was no statistical significance (P = .06). These results suggest that the cytotoxicity of excessive NO plays a role in selective RGC loss in glaucoma.
Dr. C.K. Park, Department of Ophthalmology, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, Seoul, 137-701, South Korea. ckpark@catholic.ac.kr
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
