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Abstract #19728 Published in IGR 9-4
Adenovirus-mediated gene transfer of MMP-2 into cultured porcine trabecular meshwork cells
Hayama F; Fukuchi T; Seki M; Matsuda H; Ueda J; Abe HActa Medica et Biologica 2007; 55: 81-86
This study aimed to use adenoviral gene transfer to express matrix metalloproteinase (MMP)-2 in cultured porcine trabecular meshwork cells and to evaluate the duration of adenovirus-mediated MMP-2 expression and its enzymatic activity. MMP-2 cDNA was synthesized by ligating three segments of MMP-2 cDNA obtained by reverse transcription-polymerase chain reaction (RT-PCR) with mRNA extracted from mouse lungs. MMP-2 cDNA was inserted into replication-deficient adenoviral vectors. Western blotting revealed that MMP-2 was highly expressed by adenoviral gene transfer in cultured porcine trabecular meshwork cells. Zymography confirmed that the expressed MMP-2 possessed enzymatic activity and that MMP-2 activity increased dose-responsively with the viral titer. MMP-2 expression was detected two days after the additional virus preparation and continued for at least three weeks. Adenoviral vectors could efficiently deliver MMP-2 cDNA to cultured trabecular meshwork cells, with MMP-2 gene expression persisting for three weeks after infection. Our data have implications for future gene therapy in glaucoma.
Dr. T. Fukuchi, Division of Ophthalmology and Visual Science, Graduate School of Medical and Dental Sciences, Niigata University, Asahimachi 1-757, Chuo-ku, Niigata 951-8510, Japan
Classification:
11.9 Gene therapy (Part of: 11 Medical treatment)
2.5.1 Trabecular meshwork (Part of: 2 Anatomical structures in glaucoma > 2.5 Meshwork)
