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Abstract #19831 Published in IGR 9-4
Establishment and appraisal of glaucoma animal models with chronic intraocular hypertension
Ma J-Z; He X-G; Xie L; Xi J-T; Sun Y-L; Wang Y-T; Long Z-Y; Li Y-YInternational Journal of Ophthalmology 2007; 7: 951-955
AIM: To develop a distinctive model of the chronic glaucoma with optic neuropathy induced by intraocular hypertension in rats. METHODS: The rat model with chronic elevation of intraocular pressure (IOP) was established and the changes of optic nearopathy were observed. A diode laser with wavelength of 532-nm was used to burn the trabecular meshwork and episcleral veins of right eyes at 45 SD rats, while the left eyes were referred as control. Tono-pen XL Tonometer was used to measure IOP at 3 days; one week; one, two, three and six months under Pentobarbital Sodium anesthesia. The F-VEP was monitored at the model of rats in two and six months after laser burning. The retina and optic nerve samples were obtained for morphological observation to judge whether the animal model was successful. RESULTS: Thirty-eight rat models with intraocular hypertension were obtained successfully. The IOP of experimental eyes in the morning was maintained at 25.0 ± 4.1mmHg in the first month. It was increased in all eyes to higher than the control eyes, the mean IOP of which was 17.1& plusmn; 3.2 mmHg, and then it decreased after three months. The mean IOP was the same as that in the control in six months. CONCLUSION: The rat model with chronic elevation of IOP caused by a laser injury to the trabecular meshwork represents a useful and efficient experiment model of rats with glaucoma. LA: Chinese
Dr. X.-G. He, Department of Ophthalmology, Daping Hospital, Third Military Medical University, Chongqing 400042, China. Xiangge_he@hotmail.com
Classification:
5 Experimental glaucoma; animal models
