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Abstract #19834 Published in IGR 9-4
Effect of anti-glaucoma topical medications on expression of TGF-β and MMP of human Tenons fibroblast
Zhang J-Y; Leng F; Peng S-MInternational Journal of Ophthalmology 2007; 7: 1294-1297
AIM: To evaluate the effect of anti-glaucoma topical medications on the expression level of TGF-β and MMP of cultured human Tenon's fibroblast (HTF) of glaucoma patients. METHODS: Human Tenon's fibroblast (HTF) culture media were established from explant culture of primary open-angle glaucoma (POAG) patients and of control group. RT-PCR was used to detect the mRNA level of three TGF-β isoforms and three MMP isoforms in HTF of POAG patients, compared with the cells from healthy donors. The expression level of TGF-β1 in cultured HTF before and after topical medication in vitro was evaluated by ELISA. RESULTS: All three isoforms of TGF-β were expressed on mRNA level by HTF. The expression on mRNA level of TGF-β2 and TGF-β3 was significantly higher in HTF from glaucoma patients compared with that from control group. Compared with controls, the expression level of TGF-β1 on protein level was significantly higher in glaucoma patients (P < 0.01). The expression level of TGF-β1 in cultured Tenon's fibroblast was about four times enhanced after treatment of anti-glaucoma topical medications, both in HTF from glaucoma patients and in HTF from controls (P < 0.01). MMP-2 and MMP-9 were both expressed on mRNA level by HTF. The expression on mRNA level of MMP-9 was significantly lower, about one percent of 0.1, in HTF from glaucoma patients compared with that from controls. There is no difference between two groups on mRNA level of MMP-2. MMP-3 was slightly expressed by HTF in control group, while it was almost not found in POAG group. CONCLUSION: The enhanced expression on mRNA level and protein level of TGF-β in vitro and in vivo might support the proliferation of fibroblast in glaucoma patients; the absence of MMP-3 and the reduced expression of MMP-9 on mRNA level might lead to the fibrosis of conjunctiva. All of those might result from prior use of topical medications, which were administered by all glaucoma patients in this study for at least one year and might have a positive effect on wound healing after antiglaucoma filtration surgery and influence the result of operation. LA: Chinese
Dr. S.-M. Peng, Ophthalmic Center, Hospital of Harbin Medical University, Harbin 150086 Heilongjiang Province, China. psm06511@yahoo.com
Classification:
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
11.16 Vehicles, delivery systems, pharmacokinetics, formulation (Part of: 11 Medical treatment)
12.1 General management, indication (Part of: 12 Surgical treatment)
11.1 General management, indication (Part of: 11 Medical treatment)