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Abstract #19963 Published in IGR 9-4
Molecular analysis of neurolysin expression in the rat and bovine ciliary body
Bertazolli-Filho R; Coca-Prados M; Haddad A; Laicine EMCurrent Eye Research 2007; 32: 751-756
PURPOSE: This paper deals with the capability of the ciliary epithelium to express neurolysin, involved in the inactivation of numerous neuropeptides. METHODS: Total RNAs from ciliary body (CB) were processed for RT-PCR, and the amplification products were sequenced. The whole-protein extracts of CBs were analyzed using the Western blot. The CBs were processed for neurolysin immunolocalization. RESULTS: The RT-PCR detected the presence of neurolysin mRNA in the ciliary body. The Western blot assays demonstrated immunochemical cross-reactivity with neurolysin. The immunoreactivity to neurolysin was observed in ciliary epithelium. CONCLUSIONS: These results indicate that the ciliary epithelium expresses neurolysin.
Dr. R. Bertazolli-Filho, Departamento de Biologia Celular e Molecular e Bioagentes Patogêcos, Faculdade de Medicina de Ribeirão Preto/USP, 14049-900 Ribeirão Preto, SP, Brazil. rbertazo@netscape.net
Classification:
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
2.9 Ciliary body (Part of: 2 Anatomical structures in glaucoma)