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Abstract #20109 Published in IGR 9-4
Longitudinal evaluation of retinal ganglion cell function and IOP in the DBA/2J mouse model of glaucoma
Saleh M; Nagaraju M; Porciatti VInvestigative Ophthalmology and Visual Science 2007; 48: 4564-4572
See also comment(s) by Christopher Bowd •
PURPOSE: To characterize progressive changes of retinal ganglion cell (RGC) function and intraocular pressure (IOP) in the DBA/2J mouse model of spontaneous glaucoma. METHODS: Serial pattern electroretinograms (PERGs) and IOPs measures were obtained from both eyes of 32 anesthetized DBA/2J mice over an age range of two to 12 months at one-month intervals. Cone-driven flash-ERGs (FERGs) were also recorded. The endpoint was defined as the age at which the PERG amplitude reached the noise level in at least one eye. At that point, both eyes were histologically processed to evaluate the thickness of the retinal fiber layer (RNFL). RESULTS: IOP increased moderately between two and six months ( approximately 14-17 mmHg) and then more steeply, until it leveled off at approximately 28 mmHg by nine to 11 months. The mean PERG amplitude decreased progressively after three months of age to reach the noise level (85% reduction of normal amplitude) at approximately nine to 12 months in different animals. When the PERG was at noise level, the RNFL showed a relatively smaller reduction (40%) in normal thickness. The FERG displayed minor changes throughout the observation period. IOP and PERG changes were highly correlated (r2 = 0.51, P < 0.001). CONCLUSIONS: Results indicate that inner retina function in DBA/2J mice progressively decreases after three months of age, and it is nearly abolished by ten to 11 months, whereas outer retina function shows little change and the RNFL thickness is relatively spared. This result suggests that surviving RGCs may not be functional. Progression of inner retinal dysfunction is strongly associated with increased IOP.
Dr. M. Saleh, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
6.7 Electro-ophthalmodiagnosis (Part of: 6 Clinical examination methods)
