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Abstract #21143 Published in IGR 10-2
Measurement of mouse intraocular pressure by microneedle method
Guo X; Li Y; Hu Y; Wu LChinese Ophthalmic Research 2008; 26: 165-168
OBJECTIVE: Mouse is a particularly attractive model for glaucoma research at present. Technical difficulty associated with the measurement of intraocular pressure (IOP) in mouse eyes has, however, limited the use of these animals in certain experimental paradigms. This study was taken to validate the accuracy of microneedle method and measurement of the IOP in 5 kinds of inbred mice. METHODS: Five different kinds of inbred mice including 20 mice of C3H/HeJ, C57BL6J, BALB/CJ and ICR and 7 DBA/2J mice were selected for the study. The age of the mice was 3, 6, 9 and 11 months. The animals were raised at the cycle of 14 hours of light environment and 10 hours of night environment for two weeks. The IOP of the mice was measured with a very fine fluid-filled glass microneedle device connecting ocular anterior chamber of mouse. The accuracy of IOP measured with the microneedle system was validated by adjusting the height of a water column connected to the mouse vitreous body. RESULTS: The IOP readings generated by microneedle device were correlated with the actual pressure in the mouse eye (r = 0.99, P = 0.000). The IOP value obtained by microneedle device was 10.64 ± 1.92, 10.81 ± 1.84, 10.98 ± 1.3 and 11.60 ± 1.29 mmHg respectively in C3H/HeJ, C57BL6J, BALB/CJ and ICR mouse. At 3-, 6-, 9- and 11-month-old DBA/2J mouse, the IOP was 11.43 ± 1.84, 10.54 ± 2.49, 19.35 ± 7.49 and 26.76 ± 4.4 mmHg, respectively. The fraction of eyes with IOP < 15 mmHg was 100%, 93%, 38% and 0 respectively and that with IOP > 20 mmHg was 0, 0, 38% and 91%. CONCLUSION: Microneedle measurement system is an accurate method for measurement of mouse IOP. There is no significant difference of IOP in five different kinds of 3-month-old inbred mice. The IOP presents an increase value in 9- and 11-month-old DBA/2J mice. LA: Chinese
Dr. X. Guo, Peking University Third Hospital, Peking University Eye Center, Beijing 100083, China
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
6.1.1 Devices, techniques (Part of: 6 Clinical examination methods > 6.1 Intraocular pressure measurement; factors affecting IOP)
