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Abstract #21146 Published in IGR 10-2
Protection of endogenous CNTF on retina in rat with glaucoma
Zhang M; Wu Q; Song B; Lu B; Hu PChinese Ophthalmic Research 2008; 26: 96-99
OBJECTIVE: Nowadays ciliary neurotrophic factor (CNTF) is believed to promote the growth and development and stimulate the regeneration of axons of retinal ganglion cells (RGCs) and therefore consequently obtain a great deal of attention in the field of ophthalmology. The objective of this study was to detect the expression of CNTF protein in the retina of glaucoma model with increased intraocular pressure (IOP). METHODS: The glaucoma model was created by electrocoagulating at least 3 episcleral and limbal veins in 36 adult Sprague-Dawley rats. The rats were divided into pseudosurgery group (6 rats) and chronic hypertention group (30 rats). IOP was measured in 1,3,7,14 and 28 days after modeling. The location and expression of CNTF protein were detected at 1,3,7,14 and 28 days after surgery by immunohistochemistry assay and Western blot analysis. RESULTS: The IOP was moderately elevated after modeling, showing a significant difference compared with pseudosurgery group in various time points (P < 0.01). A minimum protein expression of CNTF was found only in the ganglion cell layer of the retinas of the pseudosurgey group. In model group, CNTF was expressed in retinal inner nuclear layer and outer nuclear layer. The expression level of CNTF reached peak in the 7th day after surgery and reduced 28 days later. CONCLUSION: The increased protein expression of endogenous CNTF in the retina of a rat glaucoma model is closely linked to the axonal reaction of RGCs after the induction of ocular hypertension. LA: Chinese
Dr. M. Zhang, Department of Ophthalmology, No. 6 People's Hospital, Shanghai 200233, China. wyan559@hotmail.com
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
11.8 Neuroprotection (Part of: 11 Medical treatment)
