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Abstract #21147 Published in IGR 10-2
Study on TNF-α-mediated apoptosis of retina ganglion cell
Cui X; Wang J; Wang DChinese Ophthalmic Research 2008; 26: 108-112
OBJECTIVE: Apoptosis is the main way in the gradually losing of retina ganglion cells (RGCs) in glaucoma. Recently researches proved that TNF-α was key factor in RGCs apoptosis. The aim of this study was to investigate TNF-α-induced apoptosis of RGCs and detect the expression of apoptosis-related genes, bcl-2 and bax. METHODS: Thirty Wistar rats were randomly divided into five experimental groups and one control group. 2 μL TNF-α at 2.5, 5, 10, 20, 40 μg/mL concentration was injected into the vitreous cavity of rats in experimental groups respectively, and normal saline was used to the control group. After 2 weeks of vitreous injection, experimental rats were sacrificed and the retinal tissues were prepared for the examination of light and electronic microscopy. RESULTS: No obvious histological alteration was seen, and the almost normal cellular organs were also found in 2.5 and 5 μg/mL of TNF-α groups. The alignment of RGCs became disordered and the distance among the cells was widened, and the abnormalities of chromatin, nuclear membrane and mitochondrion were also found in 10, 20 and 40 μg/mL of TNF-α groups, however, no necrosis and inflammatory cell infiltration were observed in every group. The brown-yellow staining of bcl-2 and bax in cytoplasm of RGCs were identified in over 5 μg/mL of TNF-α groups, and the mean optical density value of bcl-2 and bax in RGCs layer was gradually enhanced with the increase of TNF-α concentration over 5 μg/mL of TNF-α groups compared with control group (P < 0.05-0.01). CONCLUSION: Over 10 μg/mL of TNF-α mediates RGCs apoptosis within 2 weeks in a concentration-dependent manner. Bcl-2 and bax participate in the apoptosis mechanism of RGCs induced by TNF-α. LA: Chinese
Dr. X. Cui. Medical School Hospital of Qingdao University, Qingdao 266003, China
Classification:
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
