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Abstract #21511 Published in IGR 10-3
Ultrastructure of rabbit model of high intraocular pressure
Lei X; Wei P; Li J; Li X; Lei J; Yang KChinese Ophthalmic Research 2008; 26: 280-284
OBJECTIVE: High intraocular pressure (IOP) directly leads to the optic nerve atrophy and therefore loss of visual function. This study was to observe the relavant parameter changes of chronic high IOP model induced by compound carbomer in rabbits and study the ultrastructure change of chronic high IOP eyes. METHODS: The chronic hypertension model was established in the left eyes of 32 healthy pigmented rabbits by the injection of 0.3 mL of 0.3% carbomer into anterior chamber, and the PBS was injected at the same way in the right eyes. The model eyes were randomly divided into 8 groups according to the different treating time. The experimental rabbits were sacrificed in 1 week, 2, 3, 4, 6, 8, 10 and 12 weeks for the histological examination under the electron microscope. Retinal single cell suspension was taken and flow cytometry assay was used for the quantitative analysis of apoptosis and necrosis. RESULTS: With drug-induced extension of time, the slow increase of IOP, retinal thickness, increased neuronal cell death were found. High IOP lasted about 3 months. The average IOP was 27-38 mmHg with the top IOP 37-45 mmHg. The successful rate of glaucoma model was 95.7%. The various layers of retina showed the different degree of significantly thinner, and the density of retinal ganglion cells (RGCs) and the thickness of retinal nerve fiber layer (RNFL) reduced by 57.8% and 66.7% in the hypertention eyes. The main form of retinal nerve cells death was apoptosis, and no significant change was seen in the proportion of cells necrosis in high IOP eyes. CONCLUSION: Compound carbomer induces moderate, stable, slow and lasting-time hypertention in rabbits with a similar pathologic characteristics in optical nerve and retina of human glaucoma. LA: Chinese
Dr. X. Lei, Department of Ophthalmology, Medical College, Lanzhou University, Lanzhou 730000, China
Classification:
5.3 Other (Part of: 5 Experimental glaucoma; animal models)
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
3.2 Electron microscopy (Part of: 3 Laboratory methods)
