advertisement
Abstract #21546 Published in IGR 10-3
Molecular analysis of CHX10 and MFRP in Chinese subjects with primary angle-closure glaucoma and short axial length eyes
Aung T; Lim MCC; Wong TTL; Thalamuthu A; Yong VHK; Venkataraman D; Venkatraman A; Chew PTK; Vithana ENMolecular Vision 2008; 14: 1313-1318
PURPOSE: The genetic basis of primary angle-closure glaucoma (PACG) has yet to be elucidated. Ocular characteristics related to PACG such as short hyperopic eyes with shallow anterior chambers suggest the involvement of genes that regulate ocular size. CHX10, a retinal homeobox gene associated with microphthalmia, and MFRP, the membrane-type frizzled-related protein gene underlying recessive nanophthalmos, represent good candidate genes for PACG due to the association with small eyes. To investigate the possible involvement of CHX1O and MFRP in PACG, we sequenced both genes in PACG patients with small ocular dimensions. METHODS: One hundred and eight Chinese patients with axial lengths measuring 22.50 mm or less were selected for analysis. Ninety-three age- and ethnically-matched control subjects were also screened. Genomic DNA was extracted from leukocytes of peripheral blood samples, and the exons of CHX1O and MFRP were amplified by polymerase chain reaction (PCR) and subjected to bidirectional sequencing and analysis. RESULTS: All study patients were Chinese with a mean age of 66.2 ± 9.1 years (range 46-86). There were 77 females (71.3%). Forty-nine out of the one hundred and eight subjects had previous symptomatic PACG, and 59 had asymptomatic PACG. The mean axial length was 21.90 ± 0.50 mm (range 19.98-22.50 mm). We identified a possible disease-causing variant in CHX1O (c.728G>A) resulting in Gly243Asp substitution in one patient. This variant was not found in 215 normal controls. Several CHX1O and MFRP polymorphisms were also identified. CONCLUSIONS: Our results do not support a significant role for CHX1O or MFRP mutations in PACG.
Dr. T. Aung, Singapore National Eye Centre, 11 Third Hospital Avenue, Singapore 168751, Singapore. tin11@pacific.net.sg
Classification:
9.3.2 Chronic primary angle closure glaucoma (pupillary block) (Part of: 9 Clinical forms of glaucomas > 9.3 Primary angle closure glaucomas)
3.4.2 Gene studies (Part of: 3 Laboratory methods > 3.4 Molecular genetics)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
