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Abstract #21737 Published in IGR 10-3
K+ currents fail to change in reactive retinal glial cells in a mouse model of glaucoma
Bolz S; Schuettauf F; Fries JE; Thaler S; Reichenbach A; Pannicke TGraefe's Archive for Clinical and Experimental Ophthalmology 2008; 246: 1249-1254
PURPOSE: To investigate the membrane physiology of Müller glial cells from retinae of DBA/2J mice (which develop ocular hypertension) and of C57BL/6 control mice of different ages. METHODS: Retinae were obtained at the ages of 3, 6, and 12 months from DBA/2J mice and from C57BL/6 controls. Immunohistochemistry was performed using antibodies against glial fibrillary acidic protein (GFAP). Whole-cell membrane currents, membrane potentials and capacitances were recorded from freshly isolated Müller cells. RESULTS: Strong immunostaining for GFAP was found in Müller cells of 12-month-old DBA/2J mice, whereas only astrocytes were immunopositive in C57BL/6 retinae. No significant alterations of membrane currents or potentials of Müller cells from DBA/2J mice as compared to controls were observed at any age; however, the membrane capacitance was increased in Müller cells from DBA/2J mice at the age of 6 months. CONCLUSIONS: Although Müller cells of DBA/2J mice display some symptoms of reactive gliosis, the lack of significant alterations of the membrane physiology confirm previous data demonstrating that these cells undergo a nonproliferative gliosis.
Dr. S. Bolz, Department of Ophthalmology, Institute for Ophthalmic Research, University of Tübingen, Röntgenweg 11, 72076, Tübingen, Germany
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
3.9 Pathophysiology (Part of: 3 Laboratory methods)