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Abstract #22160 Published in IGR 10-4
Regenerative capacity and influential factors of corneal endothelial cells
Chen G-Y; Chen G-QJournal of Clinical Rehabilitative Tissue Engineering Research 2008; 12: 6105-6108
BACKGROUND: Primary and secondary corneal endothelial decompensation is one of the crucial factors that can greatly influence the success rate of sight rehabilitating operation, such as corneal transplantation, cataract extraction, and glaucoma filtration surgery. The pathogenesis of cortical endothelial decompensation is that division of corneal endothelial cells either does not occur or occurs at a rate too slow to adequately replace dead cells throughout life. OBJECTIVE: To summarize the present studies and the influential factors of the regenerative capacity of corneal endothelial cells in order to provide a new idea for preventing and treating keratopathy. RETRIEVAL STRATEGY: The relevant articles between January 1973 and December 2007 were recruited from PubMed by using the key words 'corneal endothelial cells, regeneration, proliferation, gene transfer' in English. The China Journal Full-text Database (CJFD) was simultaneously searched for relevant articles published from January 1973 to December 2007 in Chinese with the same key words. In total, 246 articles were retrieved (185 Chinese and 61 English). All retrieved articles were screened in accordance with the topic and research objective of this article. Inclusive criteria: (1) articles with strong points and high correlation; and (2) articles in the same field published recently or in the authorized journals. Exclusive criteria: repetitive or old studies. Finally, 32 articles were included. LITERATURE EVALUATION: All articles were related to the regenerative capacity, proliferation or mitotic capacity of corneal endothelial cells, and had been published in main medical journals. Six of the selected articles were reviews. The remaining was clinical studies or experimental studies. Data synthesis: (1) The mitotic capacity of corneal endothelial cells varies from one kind of animals to another kind of animals. The characteristics of regenerative capacity for human corneal endothelial cells are as follows. One is that corneal endothelial cells exhibit intrinsic, age-related differences, and region-related differences in relative proliferative capacity. The other is that the proliferative capacity of cells is disparity between in situ and in vitro. (2) Contact inhibition, transforming growth factor (TGF)-β-2 and p27kip1 are important reasons for losing mitotic capacity of adult corneal endothelial cells. (3) Replication in corneal endothelial cells can be induced by gene transfer, growth factor and extracellular matrix in vitro. Therefore, it indicates the study prospect for prevention and cure of human cortical diseases in vivo. CONCLUSION: Regulating the growth cycle of cells in vitro can improve the regenerative capacity of corneal endothelial cells. It confers significant potential for new treatment of human corneal diseases, and may heighten the success rate of sight rehabilitating operation. LA: Chinese
Dr. G.-Y. Chen, Department of Opthalmology, Medical College, Jinggangshan University, Ji'an 343000 Jiangxi Province, China. chengenyun2007@sina.com
