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Abstract #22433 Published in IGR 10-4
Effect of Rosiglitazone on the proliferation and the production of transforming growth factor-β-1 in cultured human Tenon's capsule fibroblasts
Zheng KK; Yu MB; Jiang RZ; Li QNZhonghua Yan Ke Za Zhi 2008; 44: 413-417
OBJECTIVE: To investigate the effect of Rosiglitazone on the proliferation and the production of transforming growth factor-β-1 (TGF-β-1) in cultured humans Tenon's capsule fibroblasts (HTFs). METHODS: It was a experimental study. HTFs were cultured and subcultured in vitro. After HTFs were treated with different concentrations of Rosiglitazone, the proliferation was detected by MTT assay. The migration ability was detected by a scratch method. The production of TGF-β 1 was determined by ELISA. TGF-β-1 mRNA expression was examined by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Compared with the control group, Rosiglitazone significantly (F = 178.293, P < 0.01) inhibited the proliferation (0.33 ± 0.01 vs 0.29 ± 0.02) and the migration of HTFs in a dose-dependent manner with the concentrations ranged from 20 to 500 mg/L. Rosiglitazone also significantly (F = 86.404, P < 0.01) reduced the production of TGF-β-1 in HTFs (323.48 ± 13.69 vs 267.48 ± 20.31). TGF-β-1 mRNA was significantly (F = 176.102, P < 0.01) down-regulated (0.69 ± 0.02 vs 0.55 ± 0.02). CONCLUSIONS: This study suggests that the inhibition of the proliferation of Tenon's capsule and the migration of HTFs by Rosiglitazone is due, at least in part, to decrease the production of TGF-β-1. LA: Chinese
Dr. K.K. Zheng, Shan Tou Central Hospital, Shantou 510031, China