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Abstract #22713 Published in IGR 11-1
Detection of optic nerve head neural canal opening within histomorphometric and spectral domain optical coherence tomography data sets
Strouthidis NG; Yang H; Fortune B; Downs JC; Burgoyne CFInvestigative Ophthalmology and Visual Science 2009; 50: 214-223
PURPOSE: To assess the ability to detect the neural canal opening (NCO) and its characteristics within three-dimensional (3-D) histomorphometric and 3-D spectral domain optical coherence tomography (SD-OCT) reconstructions of the optic nerve head from nonhuman primate (NHP) eyes. METHODS: NCO was delineated within 40 radial, sagittal sections of 3-D histomorphometric reconstructions of 44 normal eyes of 38 NHPs, each perfusion fixed at IOP 10 mmHg, and 3-D SD-OCT (Spectralis; Heidelberg Engineering, Heidelberg, Germany) volumes acquired in vivo from a separate group of 33 normal eyes of 24 NHPs. Within all reconstructions, a least-squares ellipse was fitted to the 80 NCO points. For each eye, the dimensions and plane error (a gauge of planarity) of the fitted ellipse were calculated. RESULTS: The NCO was successfully delineated within every section of each histomorphometric and SD-OCT reconstruction. Median plane error was similar within histomorphometric and SD-OCT volumes (8 μm, range 4-19, histomorphometry, and 10 μm, range 4-26, SD-OCT) and was small relative to the size of the ellipse. Median histomorphometric ellipse dimensions were 1453 μm (major axis, range 1218-1737) and 1066 μm (minor axis, range 808-1263). Median SD-OCT ellipse dimensions were 1512 μm (major axis, range 1191-1865) and 1060 μm (minor axis, range 772-1248). CONCLUSIONS: The NCO is biologically continuous and relatively planar within all 3-D histomorphometric and SD-OCT reconstructions. These characteristics support its further evaluation as a reference plane for cross-sectional and longitudinal measurement of optic nerve head structures using 3-D SD-OCT.
Dr. N.G. Strouthidis, Optic Nerve Head Research Laboratory, Devers Eye Institute, Legacy Health System, Portland, OR 97208-3950, USA
Classification:
6.9.2.2 Posterior (Part of: 6 Clinical examination methods > 6.9 Computerized image analysis > 6.9.2 Optical coherence tomography)
