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Abstract #22908 Published in IGR 11-1
Expression of heat shock protein mRNA in rat retina with ocular hypertension
Li Y; Zhao W; Zeng Y; Dai L; Lino L; Ma JChinese Ophthalmic Research 2008; 26: 766-769
OBJECTIVE: Studies showed that expression of HSPs is up-regulated in cellular stress reaction, hut its expression in glaucomatous optic neuropathy is unclear. Focus of present study was to detect the changes of expression of heat shock protein (HSP27, HSP70 and HSP90) mRNA in retina of rat with ocular hypertension and explore the potential relationship between HSPs with glaucomatous optic neuropathy. METHODS: Ocular hypertention model was established in the right eyes of 15 Wistar rats by bipolar electrocoagulation condensate 3 episcleral and limbal veins, and other right eyes of 15 Wistar rats were as sham control group. The left eyes of all animals were assigned to blank control group. Intraocular pressure (IOP) was monitored twice weekly with Tonopen. In the model eyes, IOP ranged from 27 to 35 mmHg throughout the study period. In sham control eyes, IOP ranged from 12 to 17 mmHg. Rats were euthanized at 10,20, or 60 days following the induction of ocular hypertension, and RNA were isolated from retina dissected free. Northern blot analysis was used to detect mRNA expressions of HSP27, HSP70, HSP90 rat retina. RESULTS: Northern blot analysis demonstrated that compared with sham goup, expression of HSP27 mRNA in the retina showed a dynamic increase on 10,20 and 60 days following the induction of ocular hypertension with a mean elevated percentage of 250% in model group, and expression of HSP70, HSP90 mRNA followed a parallel pattern with a mean elevated percentage of 98% and 82. 6%, respectively. No significant change was found in HSP70 protine expression. CONCLUSION: The mRNA level of HSP27 is upregulated in retina of Wistar rats with ocular hypertension. There may be a potential relationship between the upregulation of HSP27 and degeneration of retinal ganglion cells in hypertension eyes. LA: Chinese
Dr. Y. Li. Department of Ophthalmology, Affiliated First Hospital, Kunming Medical College, Kunming 650032, China
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
