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Abstract #22910 Published in IGR 11-1
Induction of experimental iri neovascularization by ocular anterior segment ischemia in rabbit
Hongbo C; Fangwei Y; Feng Z; Hua WChinese Ophthalmic Research 2008; 26: 901-903
OBJECTIVE: It is important to research the new vessels glaucoma by use of iri neovascularization model. This study was to establish a kind of experimental iri neovascularization model in rabbit. METHODS: The model of iri new blood vessel was established in 5 pigmented rabbits by cutting down four extraocular rectus muscles from lateral eyes and suturing them on the conjunctiva to induce the ischemia of ocular anterior segment, and the normal fellow eyes were as contrasts. The inflammatory reaction and new vessels were observed by slit-lamp everyday, and on the 7th day, fluorescence angiography of ocular anterior segment was performed by injection of 10% fluorescein sodium to observe the leakage of new vessels on the iris. Level of vascular endothelial growth factor( VEGF) in aqueous humor was detected before and 4, 7, 11 and 14 days after operation by the ELISA. RESULTS: After operation, the conjunctiva showed congestion and aqueous fluid was unclear in the model eyes. On the 3rd day after operation, the KP and cellulose exudation were seen under the slit-lamp, and new vessels were found on the iris of operative eyes and peaked on the 7th day. The green fluorescence was seen in queous fluid immediately after injection of fluorescence, and fluorescence leakage was presented on the iris 30 seconds later. No similar change occurred in the contrast eyes. The VEGF level in aqueous humor was significantly increased on the 4th day and peaked on the 7th day and then decreased gradually on the 11th day after operation(t = 4.25, 7. 85, 16. 22, P<0. 05). On the 14th day, the VEGF level in aqueous humor was lower in model eyes than the contrast eves( t = 8. 99, P < 0. 01 ). CONCLUSION: This model can results in the ischemia of ocular anterior segment and induces the experimental neovascular effectively, and dynamic change of VEGF content in aqueous fluid shows a similar procedure to iri neovascularization. LA: Chinese
Dr. C. Hongbo, Shenzhen Eye Hospital, Affiliated Second Clinical Medical College, Jinan University, Shenzhen 518001, China
Classification:
5.3 Other (Part of: 5 Experimental glaucoma; animal models)
2.8 Iris (Part of: 2 Anatomical structures in glaucoma)
