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Abstract #23362 Published in IGR 11-1
GABA agonists fail to protect the retina from ischemia-reperfusion injury
Traustason S; Eysteinsson T; Agnarsson BA; Stefánsson EExperimental Eye Research 2009; 88: 361-366
The purpose of this study was to test the hypothesis that ischemia/reperfusion injury in the rat retina may be ameliorated by reducing retinal metabolism with either hypothermia or inhibitory GABA agonists. The intraocular pressure of each right eye in rats was raised to 130mmHg for 60min with the left eye serving as normal control. The rats were divided into four groups in terms of drug and hypothermia treatment: (1) Untreated ischemia, (2) Hypothermia, (3) Baclofen/midazolam and (4) Baclofen/muscimol. Electroretinogram was recorded before ischemia and again after 10-day reperfusion. Histological analysis with H&E staining and cell counts was performed. Untreated ischemia/reperfusion resulted in severely reduced ERG responses. The ERG b-wave was reduced from 423±144muV to 130±91muV (mean±SD, n=5). With hypothermia the ERG b-wave was reduced from 499±80muV to 237±111muV (n=4). With combinations of baclofen and midazolam the ERG b-wave was reduced from 432±96muV to 104±67muV (n=7). In baclofen/muscimol treated eyes the ERG b-wave went from 426±101muV to 148±118muV (n=6). The histological tissue damage was severe in untreated ischemia and the baclofen/midazolam and baclofen/muscimol groups, but less severe in the hypothermia group. The GABA agonists do not provide any protection in our ischemia/reperfusion model. Our results are consistent with earlier reports that hypothermia may be helpful in ischemic conditions in the retina.
Dr. S. Traustason, Department of Ophthalmology and Physiology and National University Hospital, Department of Ophthalmology and Pathology, University of Iceland, Reykjavík, Iceland
Classification:
11.8 Neuroprotection (Part of: 11 Medical treatment)
11.14 Investigational drugs; pharmacological experiments (Part of: 11 Medical treatment)
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
