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Abstract #23617 Published in IGR 11-2
Neuroprotection of recombinant human erythropoietin on chronic ocular hypertension induced retinal neuron injury in rats
Gao L-M; Duan X-C; Hu M-MInternational Journal of Ophthalmology 2009; 9: 680-683
AIM: To evaluate the neuroprotective effect and its possible mechanism of recombinant human erythropoietin (rHuEPO) in chronic ocular hypertension model of Sprague-Dawley (SD) rats. METHODS: Thirty healthy SD rats were randomly divided into five groups, which were damaged group I, damaged group II, DMSO group, rHuEPO group and rHuEPO + Wortmannin (WM) group respectively. Each group has six rats, the right eyes were experimental eyes. The rats in damaged group II and in other groups were sacrificed in one day and in eight weeks after laser treatment respectively. Chronic ocular hypertension model was induced by episcleral venous occlusion through 532nm laser for rats in damaged group and the Tono-pen was employed to measure intraocular pressure (IOP). DMSO, rHuEPO and rHuEPO + WM were chosen for intravitreal injection for each group, respectively, and intravitreal injection started from the first day after laser treatment and for once each week. The left eye of the damaged group was served as normal group. The change of EPOR, AKT and PAKT expression for each group was characterized in retinal ganglion cell layer (RGCL) by immuneohistochemistry. The amount of neuron apoptosis in RGCL was measured by TUNEL staining. RESULTS: Compared with the normal group, EPOR expression of each group significantly increased after chronic ocular hypertension (P < 0.05). Compared with the normal group, AKT expression of each group didn't change significantly, PAKT expression of the damaged group I and DMSO group either didn't change significantly, but PAKT expression of rHuEPO group increased remarkably (P < 0.05). Compared with the damaged group I and DMSO group, The amount of neuron apoptosis of rHuEPO group decreased obviously (P < 0.05). Compared with rHuEPO group, AKT expression didn't change significantly, PAKT expression decreased remarkably (P<0.05), and the amount of neuron apoptosis increased observably (P<0.05) for rHuEPO + WM group. CONCLUSION: rHuEPO has neuroprotective effect on chronic ocular hypertension induced retinal neuron injury in SD rats and it is likely that neuroprotective effect were conveyed by activation of the PI-3K/AKT signal pathway. LA: Chinese
X.-C. Duan. Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha 410011 Hunan Province, China. duanxchu@yahoo.com.cn
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
11.8 Neuroprotection (Part of: 11 Medical treatment)
